The Alternative Library

Much time and effort has been devoted to designing and developing library Web sites that are easy to navigate by both new students and experienced researchers. In a review of the Southampton Institute Library it was decided that in addition to updating the existing homepage an alternative would be offered. Drawing on theory relating to user interface design,learning styles and creative thinking, an Alternative Library navigation system was added to the more traditional library homepage. The aim was to provide students with a different way to explore and discover the wide range of information resources available by taking a less formal approach to navigation based on the metaphor of physical space and playful exploration

Spreading the Creativity Bug

This paper is a reflection on the authors’ experience of attempting to apply creative thinking techniques in the workplace following attendance at a creative thinking course. It explores the process which involved, initially, the application of the techniques to a specific project, growing awareness of the relevance to other aspects of working life, and finally the realisation of the importance of the creative thinking approach to professional development in general

Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC

Protein secretion and membrane insertion occur through the ubiquitous Sec machinery. In this system, insertion involves the targeting of translating ribosomes via the signal recognition particle and its cognate receptor to the SecY (bacteria and archaea)/Sec61 (eukaryotes) translocon. A common mechanism then guides nascent transmembrane helices (TMHs) through the Sec complex, mediated by associated membrane insertion factors. In bacteria, the membrane protein ‘insertase’ YidC ushers TMHs through a lateral gate of SecY to the bilayer. YidC is also thought to incorporate proteins into the membrane independently of SecYEG. Here, we show the bacterial holo-translocon (HTL) — a supercomplex of SecYEG–SecDF–YajC–YidC — is a bona fide resident of the Escherichia coli inner membrane. Moreover, when compared with SecYEG and YidC alone, the HTL is more effective at the insertion and assembly of a wide range of membrane protein substrates, including those hitherto thought to require only YidC